Preparation of serial dilutions

Next, use a pipette to transfer 1 milliliter of the undiluted solution to one of the test tubes filled with the dilution liquid. Once you've done that, take 1 milliliter of liquid from the test tube you just filled and transfer it to the next test tube. Continue doing this until you've gone through all of the test tubes. To learn how to calculate the final dilution factor and concentration, scroll down! Did this summary help you? Yes No. Log in Social login does not work in incognito and private browsers.

Please log in with your username or email to continue. No account yet? Create an account. Edit this Article. We use cookies to make wikiHow great. By using our site, you agree to our cookie policy. Cookie Settings. Learn why people trust wikiHow. Download Article Explore this Article methods. Related Articles. Article Summary. Method 1. Determine the proper dilution liquid. The liquid that you will be diluting your substance in is very important.

Many solutions will be diluted in distilled water, but this is not always the case. If you are diluting bacteria or other cells, you will likely want to dilute in culture media. Prepare several test tubes with 9 mL of dilution liquid.

These tubes will serve as your dilution blanks. Each tube will be a fold dilution starting from the undiluted tube. Prepare a test tube with at least 2 mL of your undiluted solution. The minimum amount needed to perform this serial dilution is 1 mL of undiluted solution. If you only have 1 mL you will not have any remaining undiluted solution. Label this tube US for undiluted solution.

Whats is a solution? A solution is a homogeneous type of mixture of two or more substances. A solution has two parts: a solute and a solvent. The solute is the substance that dissolves, and the solvent is the majority of the solution.

Solutions can exist in different phases - solid, liquid, and gas. Is direct or serial dilution more accurate? The direct dilution method uses far less sample than the serial dilution method. This figure shows only the first four concentrations via direct dilution. Essential to direct dilution is the ability to accurately transfer extremely small volumes of stock solution, which is generally not possible with pipets.

Why is molarity important in real life? Molarity is important in chemistry because, it is the measurement of concentration. A molarity of a solution is the way to figure out how much of a specific element or compound has been dissolved or used in a certain amount of the solution. Molarity is the moles of solute divided bu the number of liters of a solution.

What is the process of dilution? Please enable Javascript in order to experience the full capabilities of the application. Thank you! Cancel Post Comment.

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Top 18 books for Environmental Microbiology. Reply Microbiology Techniques. Different Aseptic Techniques in Microbiology Laboratory. Close this module. Join the Newsletter. There are several benefits of performing serial dilution. Serial dilution comes in handy when the solution is too concentrated to be used in experiments or ingredients preparation. Best Answer: In serial dilution you produce a series of dilutions, usually based on a dilution by This procedure is used to identify the number of viable micro-organisms in a fixed amount of a liquid.

It can also be fairly easily modified to give results with solid substances, e. Serial dilution involves repeatedly mixing known amounts of source culture with sterilised liquid. When fixed amounts of this dilution series are mixed with an appropriate agar and incubated, then different numbers of colonies will be obtained. By working back from an easily counted plate and using the appropriate dilution factor, the number of micro-organisms in the original source culture can be calculated.

Each member of the team can take it in turns to perform the repeated sections below, and prompt others as required. Dry the outside of the bottle, and flame the top and neck area.